Generator

Part:BBa_K395603:Design

Designed by: Toshitaka Matsubara   Group: iGEM10_Tokyo_Tech   (2010-10-19)


alcohol acetyltransferase; converts butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl ace


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1695
    Illegal NotI site found at 1596
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 125
    Illegal BamHI site found at 1565
    Illegal XhoI site found at 1605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 135
    Illegal AgeI site found at 1349
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag+T7 terminator



Source

Apple

References